Phytochemical Profile, Antioxidant, Enzyme Inhibition, Acute Toxicity, In Silico Molecular Docking and Dynamic Analysis of Apis mellifera Propolis as Antidiabetic Supplement.

This study aims to identification of phytochemical profile of Apis mellifera propolis and explore potential of its anti-diabetic activity through inhibition on α-amylase (α-AE), α-glucosidase(α-GE) and finally identified the novel antidiabetic compounds from propolis. Apis mellifera propolis extract (AMPE) exhibited elevated polyphenol 33.26 ± 0.17 (mg GAE/g) and flavonoid (15.45 ± 0.13 mg RE/g), while its also indicated moderate strong antioxidant activity (EC50 793.09 ±1.94 µg/ml). This study found that AMPE displayed promising α-AE and α-GE inhibition through in vitro study. Based on LC-MS/MS screening, 18 unique AMPE compounds were identified, majorly belonging to anthraquinone and flavonoid compounds. In silico study determined that 8 compounds of AMPE compounds exhibited strong binding to α-AE, interacting to catalytic residue of ASP197. Moreover, 2 compounds exhibit potential inhibition of α-AG, by interacting to crucial amino acids of ARG315, ASP352, and ASP69. Finally, we suggested 2,7-Dihydroxy-1-(p-hydroxybenzyl)-4-methoxy-9,10-dihydrophenanthrene and 3(3-(3,4-Dihydroxybenzyl)-7-hydroxychroman-4-one as novel inhibitors of α-AE and α-GE. Notably, these compounds were initially discovered in Apis mellifera propolis, and molecular dynamic analysis confirmed their stable binding with both enzymes over 100 ns simulations. In vivo acute toxicity test reveals AMPE as a practically non-toxic product with LD50 value of 16050mg/kg.

Time-Dependent Effect of Chitosan Nanoparticles as Final Irrigation on the Apical Sealing Ability and Push-Out Bond Strength of Root Canal Obturation.

MATERIALS AND METHODS: Fifty-six premolars were used in this study and divided by two evaluations: 28 teeth for apical sealing ability and 28 others for bond strength. Each study was assigned randomly into two groups of fourteen teeth: Group-1, final irrigation with 17% EDTA; Group-2, with 0.5% chitosan nanoparticles. Each group was further divided into two groups of 7 each: Group-A, final irrigation was applied for 1 minute; Group-B, for 3 minutes. All teeth were obturated with epoxy resin-based sealer and gutta-percha. In the apical sealing ability study, the obturated teeth were immersed in 2% methylene blue and observed under a stereomicroscope (8x magnification). In the bond strength study, the teeth were tested using the push-out technique and observed under a stereomicroscope (40x magnification) to determine the failure type. Data from each evaluation were analysed with two-way ANOVA followed by the LSD test. RESULTS: Final irrigation using 0.5% chitosan nanoparticles produced the same apical sealing ability and bond strength as 17% EDTA (p > 0.05). A significant difference occurred between application times (p < 0.05). The failure type was observed predominantly as cohesive, and the least was adhesive. CONCLUSION: Regardless of the final irrigation solution used, 3-minute application time produced greater apical sealing ability and push-out bond strength than 1-minute application time.